Rice Biotechnology

Introduction

CNRRI deems Rice Biotechnology a system that contributes to the increased productivity, prosperity, well being of rice farmers and sustainability of rice farming practice. It aims to familiarize the participants with a solid theoretical grounding in plant tissue culture, rice transgenic techniques, DNA marker-aided selection and laboratory experience in relevant biotechnologies, molecular, genetic mapping, gene cloning and statistical methods.

Goals

  • Recognize and apply the important biotechnologies of rice breeding
  • Conduct experimental operations concerning tissue culture, genetic engineering
  • Obtain systematic knowledge of DNA marker-assisted selection (MAS) as well as skills for MAS related biotechnologies
  • Carry out primary studies on genes/QTLs mapping and/or cloning and researches of rice functional genome.

Duration

Six weeks

Course Content

Unit ⅠGenetic engineering

Molecular markers developing and construction of genetic maps construct
Rice transgenic technologies
Gene/QTL mapping and cloning
Rice functional genome studies

Unit ⅡRice Biotechnology

Tissue culture and anther culture techniques
Experimental techniques for rice biotechnology assisted breeding
DNA marker-assisted selection system 
DNA marker polymorphism screening and analysis

Methodology

The training approach for the course is classroom based but also includes experiments, practical illustrations and project studies undertaken in the labs of State Key Laboratory of Biotechnology and lab of China National Rice Research Institute, CNRRI. About 60% of training time is devoted to lectures, group discussions, and problem solving exercises and remaining time is centered on experiments in the laboratories.

Training Team

The course is instructed by a team of experienced CNRRI scientists:
(The names are listed in alphabetical order.)

QIAN Qian

Ph.D., Research Geneticist & Breeder

Mr. Qian’s works are dedicated to rice genetics, localization and cloning of functional genes in rice through his committed involvement with national and international cooperation projects. He has successfully cloned the MOC1, a gene controlling branching and bud formation in rice, in collaboration with Chinese Academy of Sciences (CAS). In addition, he has initiated DNA fingerprinting studies for identification of hybrid rice seeds in China, proposed and participated in the breeding of the first PGMS line with morphological marker, and developed new rice varieties with giant embryo and forage cultivars.

Mr. Qian is currently involved in innovation of rice germplasm resources and has published over 70 research articles in many leading journals such as Nature, Plant Cell and Theor Appl Genet.

SUN Zongxiu

Research Geneticist & Biotechnologist

Mr. Sun concentrates on construction of T-DNA insertion mutagenesis population, analyzing and cloning genes of interest, bioreactor studies, identification of fertility transformation reaction to photo-thermo treatments of two-line CMS lines. He has published three books and over 80 research articles.

Wu Jianli

Research Geneticist

Mr. Wu is former consultant at Asian Rice Biotechnology Network (ARBN) and program scientist of Department of Entomology and Plant Pathology, IRRI. He focuses on development of molecular markers, gene localization and cloning and modern biotechnologies like MAS. Mr. Wu has published over 50 scientific articles in journals that are peer-reviewed.

YANG Changdeng

Research Geneticist & Breeder

Mr. Yang’s research interests include construction of new rice breeding system through combination of cutting biotechnologies and conventional breeding techniques, resistant gene identification, development of breeding material conferring multiple resistance, cell culture and its utilization, breeding of early-season high quality rice varieties, etc. He has published one book and over 30 scientific articles.

ZHENG Kangle

Research Geneticist

Mr. Zheng engages in genetic background of rice, relationship investigation, localization of agronomically important genes and construction of marker-assisted selection system using molecular markers. He has inaugurated researches of genetic mechanism of high yield and heterosis, mapping and cloning of disease resistant genes at CNRRI. Mr. Zheng has published over 100 research papers in journals that are peer-reviewed.

ZHUANG Jieyun

Research Geneticist

Mr. Zhuang concentrates his research efforts currently on molecular mapping of disease resistance gene and QTLs for agronomically important traits in rice. Centering on rice genetics & breeding and biotechnologies, Mr. Zhuang has published over 70 research articles in journals that are peer-reviewed and received numerous awards and honors.

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